AEM 00084 - 12 ( Revised ) 1 Iodide Oxidation by a Novel Multicopper Oxidase from 2 α - Proteobacterium Strain Q - 1 3 4 Running title : Bacterial iodide - oxidizing enzyme 5
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AEM00084-12 (Revised) 1 Iodide Oxidation by a Novel Multicopper Oxidase from 2 α-Proteobacterium Strain Q-1 3 4 Running title: Bacterial iodide-oxidizing enzyme 5 Mio Suzuki, Yoshifumi Eda, Shiaki Ohsawa, Yu Kanesaki, Hirofumi 6 Yoshikawa, Kan Tanaka, Yasuyuki Muramatsu, Jun Yoshikawa, Ikuo Sato, 7 Takaaki Fujii, and Seigo Amachi 8 9 Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba 10 271-8510, Genome Research Center, NODAI Research Institute, Tokyo University of 11 Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Department of 12 Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 13 156-8502, Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-29 14 Nagatsuta, Midori-ku, Yokohama 226-8503, and Department of Chemistry, Gakushuin 15 University, Mejiro 1-5-1, Toshima-ku, Tokyo 171-8588, Japan. 16 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.00084-12 AEM Accepts, published online ahead of print on 23 March 2012
منابع مشابه
Iodide oxidation by a novel multicopper oxidase from the alphaproteobacterium strain Q-1.
Alphaproteobacterium strain Q-1 is able to oxidize iodide (I(-)) to molecular iodine (I(2)) by an oxidase-like enzyme. One of the two isoforms of the iodide-oxidizing enzyme (IOE-II) produced by this strain was excised from a native polyacrylamide gel, eluted, and purified. IOE-II appeared as a single band (51 kDa) and showed significant in-gel iodide-oxidizing activity in sodium dodecyl sulfat...
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Here we report the draft genome sequence of strain Q-1, an iodide (I(-))-oxidizing heterotrophic bacterium in the class Alphaproteobacteria isolated from natural gas brine water. The genome sequence contained a multicopper oxidase gene probably responsible for iodide oxidation. A photosynthetic gene cluster was found but genes for carbon-fixation were absent.
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Roseovarius sp. A-2 is a heterotrophic iodide (I-)-oxidizing bacterium isolated from iodide-rich natural gas brine water in Chiba, Japan. This strain oxidizes iodide to molecular iodine (I2) by means of an extracellular multicopper oxidase. Here we report the draft genome sequence of strain A-2. The draft genome contained 46 tRNA genes, 1 copy of a 16S-23S-5S rRNA operon, and 4,514 protein codi...
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Microorganisms catalyze the formation of naturally occurring Mn oxides, but little is known about the biochemical mechanisms of this important biogeochemical process. We used tandem mass spectrometry to directly analyze the Mn(II)-oxidizing enzyme from marine Bacillus spores, identified as an Mn oxide band with an in-gel activity assay. Nine distinct peptides recovered from the Mn oxide band of...
متن کاملAtmospheric N Deposition Increases Bacterial Laccase - Like Multicopper 1 Oxidases : Implications for Organic Matter Decay
1 Oxidases: Implications for Organic Matter Decay 2 3 Zachary Freedman and Donald R. Zak 4 5 School of Natural Resources & Environment and Department of Ecology and 6 Evolution, University of Michigan, Ann Arbor, Michigan, USA 48109 7 8 9 #Corresponding author 10 11 Address: 440 Church Street 12 Ann Arbor, MI 48109 13 e-mail: [email protected] 14 tel: (734)763-8003 15 fax: (734)763-8395 16 17 18 1...
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تاریخ انتشار 2012